Recombinant Protein Expression
and Purification

SynoGene provides a complete solution from DNA to protein in a timely manner by utilizing sophisticated platforms. SynoGene is able to provide up to gram of recombinant proteins in a shot time by transient production in mammalian cells. The yields of our expression systems are ranked the highest in the field by optimized expression systems from gene, secretion signal, expression vectors, culture conditions to purification in both mammalian cells and E. coli. SynoGene is able to generate high yield stable cell lines/pools for you.

SynoGene’s Powerful Platforms

- Super high yield of transient expression system
- High yield/titer stable cell line/pool development
- Powerful bacterial secretion system
- Reliable bacterial inclusion body refolding

Our expertise will help you overcome bottlenecks in recombinant protein expression and purification, such as difficult-to-express protein, aggregation, insolubility, etc. We provide flexible services from small-scale (shake flask) to large scale (bioreactor) production and purification. Quality is guaranteed to meet your needs from early target discovery and functional analysis to pre-clinical development. SynoGene provides outstanding services in recombinant protein expression and purification with high quality.

Expression System

- E. coli (secreted and non-secreted, soluble and insoluble)
- Mammalian cell transient expression
- Stable cell line/pool development

Our Services Include

- Gene optimization and synthesis
- Cloning and sub-cloning your target gene into our proprietary expression vectors
- Protein production in transient expression in mammalian cells
- Stable cell line/pool development in mammalian cells
- Protein production in E. coli as soluble form in secretion system, or soluble form inside the cells, or as insoluble inclusion body
- Bacterial inclusion body refolding
- Expression evaluation and optimization of expression conditions
- Mini-scale to large-scale production and purification
- Endotoxin removal (Multiple approaches, high recovery, never failed!)
- Protein characterization (SDS-PAGE, Western blot, MS, quantification, purity, IEF, aggregation, endotoxin, etc.)

We Provide Flexible Services

- Send us your own ready-to-express plasmid. We do the rest for you.
- Send us your DNA/protein sequence. We do everything from DNA to protein, including gene optimization, assembly, cell line development, production, and purification.

 

 
Contact us for a quote or further information

 

 

 


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